{"id":11426,"date":"2022-12-10T10:30:23","date_gmt":"2022-12-10T02:30:23","guid":{"rendered":"https:\/\/clbiomed.com\/?p=11426"},"modified":"2022-12-19T11:15:18","modified_gmt":"2022-12-19T03:15:18","slug":"clbs-n-1-perfusion-manufacturing-process","status":"publish","type":"post","link":"https:\/\/clbiomed.com\/2022\/12\/clbs-n-1-perfusion-manufacturing-process\/","title":{"rendered":"CLB’s N-1 perfusion manufacturing process"},"content":{"rendered":"

\"\"By Travis Pysar, Chief Operating Officer, Cheerland Biotechnology<\/em><\/p>\n

<\/section>\n
\n
Cheerland Biotechnology (CLB) has incorporated perfusion technology at the N-1 bioreactor step in both our5,000L<\/strong>\u00a0and\u00a015,000L<\/strong>\u00a0facilities. \u00a0Perfusion capabilities at the N-1 bioreactor step can be utilized for a perfusion process harvested directly from the N-1 bioreactor or the N-1 perfusion can be utilized for process intensification of the production bioreactor.<\/section>\n
<\/section>\n

Perfusion Processes<\/strong><\/h4>\n

The N-1 bioreactor can be utilized as the production bioreactor for a perfusion process or continuous manufacturing process. Perfusion processes rely on smaller bioreactor volumes where the product is harvested on a continual basis and the harvested material is replaced with fresh media. \u00a0Utilizing the smaller N-1 bioreactor as the perfusion production bioreactor decreased the media required to maintain the cell culture, thereby reducing the media prep vessel size requirements and reducing the cost of the media. \u00a0Perfusion processes can easily achieve a\u00a010 g\/L<\/strong>\u00a0titer over a typical 14-day process, thereby increasing the output compared to a standard fed-batch process.<\/p>\n

CLB has incorporated a direct transfer from the N-1 bioreactor to facilitate a perfusion process or allow for a smaller production bioreactor size for fed-batch process.<\/p>\n

\"\"<\/p>\n

 <\/p>\n

Process Intensification<\/strong><\/h4>\n

The N-1 bioreactor can also be utilized to intensify the cell culture density in the seed train prior to inoculating the production bioreactor. Similar to a perfusion process, the media is continually replaced with fresh media to support high cell densities in the N-1 bioreactor. \u00a0The production bioreactor is then inoculated with a high seed density to promote higher titers in the production bioreactor. \u00a0This process intensification can yield titers from\u00a08-10 g\/L<\/strong>\u00a0in the production bioreactor.<\/p>\n

Flexibility<\/strong><\/h4>\n

CLB is committed to meeting the needs of all of our clients, whether that is through a standard fed-batch, an N-1 perfusion batch, a N-1 fed-batch, or an N-1 process intensification. Our state of the art facilities offer the most flexibility and the highest productivity.<\/p>\n<\/article>\n","protected":false},"excerpt":{"rendered":"

offers the most flexibility and the highest productivity<\/p>\n","protected":false},"author":2,"featured_media":11439,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[104,2],"tags":[167,207],"acf":[],"yoast_head":"\nCLB's N-1 perfusion manufacturing process - \u4e50\u571f\u751f\u547d\u79d1\u6280<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/clbiomed.com\/2022\/12\/clbs-n-1-perfusion-manufacturing-process\/\" \/>\n<meta property=\"og:locale\" content=\"zh_CN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"CLB's N-1 perfusion manufacturing process - \u4e50\u571f\u751f\u547d\u79d1\u6280\" \/>\n<meta property=\"og:description\" content=\"offers the most flexibility and the highest productivity\" \/>\n<meta property=\"og:url\" content=\"https:\/\/clbiomed.com\/2022\/12\/clbs-n-1-perfusion-manufacturing-process\/\" \/>\n<meta property=\"og:site_name\" content=\"\u4e50\u571f\u751f\u547d\u79d1\u6280\" \/>\n<meta property=\"article:published_time\" content=\"2022-12-10T02:30:23+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2022-12-19T03:15:18+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/clbiomed.com\/wp-content\/uploads\/2022\/12\/1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1280\" \/>\n\t<meta property=\"og:image:height\" content=\"720\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"CLB\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"\u4f5c\u8005\" \/>\n\t<meta name=\"twitter:data1\" content=\"CLB\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u9884\u8ba1\u9605\u8bfb\u65f6\u95f4\" \/>\n\t<meta name=\"twitter:data2\" content=\"2\u5206\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/clbiomed.com\/2022\/12\/clbs-n-1-perfusion-manufacturing-process\/\",\"url\":\"https:\/\/clbiomed.com\/2022\/12\/clbs-n-1-perfusion-manufacturing-process\/\",\"name\":\"CLB's N-1 perfusion manufacturing process - 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